We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Hypoxia on Biology of Human Leukemia T-cell Line (MOLT-4 cells) Co-cultured with Bone Marrow Mesenchymal Stem Cells.
- Authors
Baharaghdam, Sina; Yousefi, Mehdi; Movasaghpour, Aliakbar; Solali, Saeed; Talebi, Mehdi; Ahani-Nahayati, Milad; Lotfimehr, Hamid; Shamsasanjan, Karim
- Abstract
Background: One of the most significant problems in the treatment of leukemia is the expansion of resistance to chemotherapeutic agents. Therefore, assessing the drug resistance and especially the drug resistance genes of leukemic cells is important in any treatment. The impact of Mesenchymal Stem Cells (MSCs) and hypoxic condition have been observed in the biological performance of majority of leukemic cells. Methods: MOLT-4 cells were co-cultured with MSCs in the hypoxic condition induced by Cobalt Chloride (CoCl2) for 6 and 24 hr. Then, apoptosis of cells was analyzed using annexin-V/PI staining and expression of the drug resistance genes including MDR1, MRP, and BCRP along with apoptotic and anti-apoptotic genes, including BAX and BCL2, was evaluated by real-time PCR. Results: The hypoxic condition for MOLT-4 cells co-cultured with MSCs could signifi- cantly increase the expression of MDR1 and BCRP genes (p<0.05) which are involved in drug resistance. Also, the results indicated that this condition significantly increases the expression of BCL2 (p<0.05) and reduces the apoptosis in MOLT-4 cells co-cultured with MSCs in the hypoxic condition. Conclusion: These effects can demonstrate the important role of hypoxia and MSCs on the biological behavior of Acute Lymphoblastic Leukemia (ALL) cells that may lead to particular treatment outcomes.
- Subjects
BONE marrow physiology; HYPOXEMIA; APOPTOSIS; CELL culture; CELL lines; COBALT; DRUG resistance; GENE expression; LYMPHOBLASTIC leukemia; POLYMERASE chain reaction; STAINS &; staining (Microscopy); STEM cells
- Publication
Avicenna Journal of Medical Biotechnology, 2018, Vol 10, Issue 2, p62
- ISSN
2008-2835
- Publication type
Article