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- Title
The heat shock protein 90 of Toxoplasma gondii is essential for invasion of host cells and tachyzoite growth.
- Authors
Hongchao Sun; Xunhui Zhuo; Xianfeng Zhao; Yi Yang; Xueqiu Chen; Chaoqun Yao; Aifang Du
- Abstract
Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects almost all warmblooded vertebrates. Heat shock proteins (HSP) regulate key signal transduction events in many organisms, and heat shock protein 90 (Hsp90) plays an important role in growth, development, and virulence in several parasitic protozoa. Here, we discovered increased transcription of the Hsp90 gene under conditions for bradyzoite differentiation, i.e. alkaline and heat shock conditions in vitro, suggesting that Hsp90 may be connected with bradyzoite development in T. gondii. A knockout of the TgHsp90 strain (DHsp90) and a complementation strain were constructed. The TgHsp90 knockout cells were found to be defective in host-cell invasion, were not able to proliferate in vitro in Vero cells, and did not show long-time survival in mice in vivo. These inabilities of the knockout parasites were restored upon complementation of TgHsp90. These data unequivocally show that TgHsp90 contributes to bradyzoite development, and to invasion and replication of T. gondii in host cells.
- Publication
Parasite (1252607X), 2017, Vol 24, p1
- ISSN
1252-607X
- Publication type
Article
- DOI
10.1051/parasite/2017023