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- Title
Changes in ACTN3 gene expression and fiber type composition in flexor hallucis longus muscle after eight weeks progressive resistance training in Sprague-Dawley rats.
- Authors
Ali Gaeini, Abbas; Khaledi, Neda; Fayazmilani, Rana; Ravasi, Aliasghar; Sedghroohi, Golnoosh; Arabkari, Vahid
- Abstract
Background: Alpha-actinins are located in the skeletal muscle Z-line and form actin-actin cross-links. It belongs to a highly conserved family of actin-binding proteins- the spectrin superfamily, which also contains the spectrins and dystrophin. Mammalian skeletal muscle has two isoforms: alpha-actinins-2 and alpha-actinins-3. However, the response of alpha-actinins to exercise training is little understood. This study examined the effects of 8 weeks of resistance training on muscle mass, ACTN3 (alpha-actinins-3) gene expression levels and fiber type composition in the flexor hallucis longus (FHL) muscle. Methods: Forty five female Sprague-Dawley rats (Initial body mass: 169.25±9gr age: 3 month) were obtained and assigned to a control (C; n=18) or exercise training (T; n=22) and pilot (P; n=5) groups. The resistance training consisted of climbing a ladder carrying a load suspended from the tail and the weight increased progressively. Real-time PCR and Immunohistochemistry techniques were used to measure gene expression levels and myosin heavy chain (MyHC) composition, respectively. Results: Following 8 weeks of training, we observed significant increase in absolute muscle mass in FHL (P=0.01). Results showed that no significant difference was found in ACTN3 gene expression levels between training and control groups (P=0.852 respectively). Also, Pearson coefficient didn't indicated any significant relationships in gene expression and Fiber type IIX in response to resistance training in FHL (r=0.12). Conclusion: However, resistance training effects on sarcomeric proteins development, these results showed no effect of resistance training on alpha-actinins-3 levels. Although alpha-actinins-3 has an important function to produce and progress of force in sarcomere, but didn't changed significantly in response to resistance training.
- Subjects
FLEXOR hallucis longus; LABORATORY rats; GENE expression; MICROFILAMENT proteins; RESISTANCE training; IMMUNOHISTOCHEMISTRY
- Publication
Tehran University Medical Journal, 2013, Vol 71, Issue 1, p37
- ISSN
1683-1764
- Publication type
Article