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- Title
Recombinant human erythropoietin enhances superoxide production by FMLP-stimulated polymorphonuclear leukocytes in hemodialysis patients.
- Authors
Hung-Chun Chen; Jer-Chia Tsai; Juei-Hsiung Tsai; Yung-Hsiung Lai
- Abstract
Recombinant human erythropoietin enhances superoxide production by FMLP-stimulated polymorphonuclear leukocytes in hemodialysis patients. Recombinant human erythropoietin (rHuEPO) is a hematopoietic growth factor that has a broad spectrum of action. We have observed the in vivo and <em>in vitro</em> effects of rHuEPO on the superoxide production of circulating polymorphonuclear leukocytes (PMNs) in hemodialysis patients. The PMNs were separated from heparinized blood after dextran sedimentation and Ficoll-Conray centrifugation and stimulated with formyl-methionyl-leucyl-phenylalanine (FMLP). serum-treated zymosan (STZ), or phorbol myristate acetate (PMA). The in vivo study showed that rHuEPO therapy for 12 weeks enhanced the superoxide production by FMLP-stimulated PMNs (<em>P</em> < 0.0]). However, no significant changes on superoxide production was found in either STZ- or PMA-stimulated PMNs. Simultaneous measurement nf PGE2 production by PMNs in response to all three stimulants did not show any significant changes after rHuEPO therapy. The direct in vitro effect of rHuEPO on PMNs showed that rHuEPO does not enhance the superoxide production by nonstimulated PMNs. However, preincubation of rHuEPO enhanced superoxide production from FMLP- and STZ-stimulated PMNs. Our results indicate thai rHuEPO enhanced FMLP-stimulated superoxide production of PMNs both <em>in vivo</em> and <em>in vitro</em> in hemodialysis patients, which may be responsible for the increased oxidant stress in hemodialysis patients after rHuEPO therapy.
- Subjects
ERYTHROPOIETIN; HEMATOPOIETIC growth factors; NEUTROPHILS; HEMODIALYSIS patients; GROWTH factors; AMINO acids
- Publication
Kidney International, 1997, Vol 52, Issue 5, p1390
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.466