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- Title
Localization of cyclophilin A and cyclophilin C mRNA in murine kidney using RT-PCR.
- Authors
Otsuka, Masakazu; Terada, Yoshio; Tianxin Yang; Nonoguchi, Hiroshi; Tomita, Kimio; Marumo, Fumiaki
- Abstract
Cyclosporin A (CsA), which is widely used as an immunosuppressant, has a nephrotoxic side effect. The mechanism of this nephrotoxicity is not well understood; however, recent studies suggest that cyclophilin (cyp) is responsible for mediating the immunosuppressive action of CsA through the interaction with the Ca2+- and calmodulin-dependent phosphatase. calcineurin. While cyp A mRNA is expressed ubiquitously, cyp C mRNA has been shown to be topically expressed, including in the kidney. We examined: (1) distribution of cyp A and cyp C mRNA in microdissected murine nephron segments, using a combination of reverse transcription and polymerase chain reaction (RT-PCR) techniques, and (2) the effect of CsA administration on cyp C mRNA expression in proximal convoluted tubule. Among the nephron segments examined, large signals for cyp C PCR product were detected in proximal convoluted tubule and proximal straight tubule. Our data showed that the distribution of cyp C mRNA was uneven, and it mainly existed in segments that are relatively sensitive to CsA toxicity. In contrast, cyp A mRNA was found to be distributed almost equally along the nephron segments examined. By CsA administration, the signal for cyp C mRNA PCR product was increased. These results suggest that cyp C may play some role in the renal tubular disorder observed in CsA nephrotoxicity.
- Subjects
CYCLOPHILINS; MESSENGER RNA; IMMUNOSUPPRESSIVE agents; NEPHROTIC syndrome; NEPHROTOXICOLOGY; POLYMERASE chain reaction
- Publication
Kidney International, 1994, Vol 45, Issue 5, p1340
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1994.175