We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Selectivity of renal injury and proteinuria in the spontaneously hypertensive rat.
- Authors
Feld, Leonard G.; Van Kiew, Judith B.; Galaske, Rainer G.; Boyland, John W.; Hui Chang; Manz, Nancy; Muir, Patricia
- Abstract
We have followed the concentration and fractional composition of tubular fluid protein and urinary protein in spontaneously hypertensive rats (SHR) from 6 to 65 weeks of their age. At intervals these functional data were correlated with the morphologic changes read by light microscopy. Matched control animals were of the same genetic strain, Wistar-Kyoto (WKY). Proximal tubular fluid protein was measured by capillary polyacrylamide gel electrophoresis; urinary protein, by Lowry method: and fractionation, by gradient gel electrophoresis. Tubular fluid protein in superficial nephrons did not change, although protein excretion rose in the SHR group to six times the control value (4.3 to 26 mg/24 hr × 100 g of body wt). The albumin fraction of urinary protein increased in the SHR from 10.5 ± 3.1% (SD) at week 6 to 72.3 ± 9.0% at week 65. No change occurred in WKY controls. Pathologic changes in SHR were strikingly limited to glomeruli and vessels of inner cortical nephrons, and progressed rapidly from 45 to 65 weeks. Glomeruli showed focal sclerosis with obliteration of the capillary tuft. Corresponding tubules wert filled with protein casts. Small arteries and arterioles showed thickening and hyperplasia, particularly in the medial layer. Periglomerular fibrosis was moderate to marked. None of these changes were found in normotensive controls. The distinguishing pattern of proteinuria (albuminuria coincident with a decrease in low mol wt protein excretion) which evolved during the first year in SHR was temporally related to glomerular and arteriolarsclerotic changes. These changes mainly affected the deep cortical nephrons. Which were less protected, perhaps, by autoregulation in the face of sustained hypertension. Superficial glomeruli were initially spared, and this is consistent with the finding of normal tubular fluid protein concentration in surface nephrons.
- Subjects
PROTEINS; LABORATORY rats; MICROSCOPY; POLYACRYLAMIDE; GEL electrophoresis; KIDNEY tubules
- Publication
Kidney International, 1977, Vol 12, Issue 5, p332
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1977.120