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- Title
Renin-Angiotensin System (RAS) Expressions in Myeloid Leukemic Cell Lines.
- Authors
UZ, Burak; TATONYAN, Suzin C.; SAYITOGLU, Muge; ERBILGIN, Yucel; HATIRNAZ NG, Ozden; BUYUKASIK, Yahya; AKSU, Salih; SAYINALP, Nilgun; GOKER, Hakan; OZCEBE, Osman I.; OZBEK, Ugur; HAZNEDAROGLU, Ibrahim C.
- Abstract
The aim of this study is to search critical renin-angiotensin system (RAS) elements in myeloid leukemic cell lines. Human acute myeloid leukemia (AML) cell lines, KG-1 and HL-60, were cultured. We searched for the gene expression of the major RAS components in KG-1 and HL-60 cell lines by quantitative real-time polymerase chain reaction analysis (qRT-PCR). Angiotensin-converting enzyme I (ACE I) and ACE II mRNA expressions were detected in KG-1 AML cell line. Relative expression of ACE I was higher than ACE II expression in this myeloid leukemic cells. Likewise, RENIN, angiotensinogen (ANGTS), and ACE I mRNA expressions were detected in HL-60 promyelocytic cell line. Relative RENIN expression was the highest, whereas ACE I expression was the lowest in HL- 60 neoplastic myeloid cells. These findings indicate that there is a biologically active local RAS in the hematopoietic system in normal and pathological states.
- Subjects
RENIN-angiotensin system; MYELOID leukemia genetics; CELL lines; GENE expression; MESSENGER RNA; HEMATOPOIETIC system
- Publication
UHOD: International Journal of Hematology & Oncology / Uluslararasi Hematoloji Onkoloji Dergisi, 2013, Vol 23, Issue 4, p264
- ISSN
1306-133X
- Publication type
Article
- DOI
10.4999/uhod.13014