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- Title
Acceleration of bone regeneration of horizontal bone defect in rats using collagen-binding basic fibroblast growth factor combined with collagen scaffolds.
- Authors
Nakamura, Shin; Ito, Takashi; Okamoto, Kentaro; Mima, Takehiko; Uchida, Kentaro; Siddiqui, Yasir D.; Ito, Masahiro; Tai, Masako; Okubo, Keisuke; Yamashiro, Keisuke; Omori, Kazuhiro; Yamamoto, Tadashi; Matsushita, Osamu; Takashiba, Shogo
- Abstract
<bold>Background: </bold>Basic fibroblast growth factor (bFGF) has been applied for periodontal regeneration. However, the application depends on bone defect morphology because bFGF diffuses rapidly from defect sites. In a previous study, collagen-binding bFGF (CB-bFGF) has been shown to enhance bone formation by collagen-anchoring in the orthopedic field. The aim of this study is to demonstrate the efficacy of CB-bFGF with collagen scaffolds in bone regeneration of horizontal bone defect.<bold>Methods: </bold>Cell proliferation activity and collagen binding activity of CB-bFGF was confirmed by WST-8 assay and collagen binding assay, respectively. The retention of CB-bFGF in the collagen sheet (CS) was measured by fluorescence imaging. The rat horizontal alveolar bone defect model was employed to investigate the efficacy of CB-bFGF with collagen powder (CP). After 4 and 8 weeks, the regenerative efficacy was evaluated by microcomputed tomography, histological, and immunohistochemical analyses.<bold>Results: </bold>CB-bFGF had a comparable proliferation activity to bFGF and a collagen binding activity. CB-bFGF was retained in CS longer than bFGF. At 8 weeks postoperation, bone volume, bone mineral content, and new bone area in CB-bFGF/CP group were significantly increased compared with those in other groups. Furthermore, epithelial downgrowth was significantly suppressed in CB-bFGF/CP group. At 4 weeks, the numbers of osteocalcin, proliferating cell nuclear antigen, and osteopontin-positive cells at the regeneration site in CB-bFGF/CP group were greater than those in other groups.<bold>Conclusions: </bold>CB-bFGF/CP effectively promoted bone regeneration of horizontal bone defect possibly by sustained release of bFGF. The potential of CB-bFGF composite material for improved periodontal regeneration in vertical axis was shown.
- Subjects
BONE regeneration; BONE abnormalities; FIBROBLAST growth factors; COLLAGEN; TISSUE scaffolds; PERIODONTITIS; LABORATORY rats; CELL proliferation; MOTION; GROWTH factors; ANIMAL experimentation; RATS; RESEARCH funding; COMPUTED tomography
- Publication
Journal of Periodontology, 2019, Vol 90, Issue 9, p1043
- ISSN
0022-3492
- Publication type
journal article
- DOI
10.1002/JPER.18-0674