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- Title
FIRST RECORD OF THE FIVE DNA BARCODES OF THE NOTHOSPECIES COCOR BEBEK (KALANCHOE × LAETIVIRENS).
- Authors
ROSLIM, D. I.; PUTRA, Y. O.; DEWI, Y. M.; BAGO, Y.; SITOHANG, H.; HERMAN; FITMAWATI; SOFIYANTI, N.
- Abstract
Cocor bebek (Kalanchoe × laetivirens) is a nothospecies. Specifically, it is a hybrid species that resulted from hybridization between two different species, namely, Kalanchoe daigremontiana and Kalanchoe laxiflora. The DNA sequences of this species are not yet available in GenBank but are important for its rapid identification. Therefore, this study aimed to isolate and analyze five DNA barcode sequences, i.e., 26S rRNA, internal transcribed spacer (ITS), trnL-trnL-trnF intergenic spacer (IGS), ndhC-trnV IGS, and rpl16 intron, of the nothospecies Kalanchoe × laetivirens. Plant samples were collected from Pekanbaru City, Riau Province, Indonesia. The DNA sequences of 26S rRNA, ITS, trnL-trnLtrnF IGS, ndhC-trnV IGS, and rpl16 intron were obtained with lengths of 898, 684, 908, 954, and 937 bp, respectively, and registered in the GenBank database with the registration numbers MW286359, MW286360, MW286361, MW286362, and MW286363, respectively. BLASTn analysis on four DNA barcodes, except for ITS, showed that Kalanchoe × laetivirens had the highest similarity to K. daigremontiana with an identity value of 97.25%-99.90%, query cover of 100%, and E value of 0.0. The DNA barcodes 26S rRNA, ITS, trnL-trnL-trnF IGS, ndhC-trnV IGS, and rpl16 intron obtained in this study revealed high variations among the studied accessions due to the presence of substitution and deleted mutations. A number of nucleotides critical for the molecular identification of this plant were generated in all of the five DNA sequences. The trnL-trnF IGS sequence produced eight critical nucleotides, and 26S rRNA and ndhC-trnV IGS each produced one. On the basis of the nucleotide similarity between the two species, the results proved that Kalanchoe × laetivirens descended from K. daigremontiana.
- Subjects
RIAU (Indonesia : Province); INDONESIA; KALANCHOE; GENETIC barcoding; DNA sequencing; DNA; PLANT identification
- Publication
SABRAO Journal of Breeding & Genetics, 2021, Vol 53, Issue 2, p263
- ISSN
1029-7073
- Publication type
Article