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- Title
Inhibitory Effect of Inflexinol on Nitric Oxide Generation and iNOS Expression via Inhibition of NF-κB Activation.
- Authors
Jae Woong Lee; Moon Soon Lee; Tae Hun Kim; Hwa Jeong Lee; Seong Su Hong; Young Hee Noh; Bang Yeon Hwang; Jai Seup Ro; Jin Tae Hong
- Abstract
Inflexinol, an ent-kaurane diterpenoid, was isolated from the leaves of Isodon excisus. Many diterpenoids isolated from the genus Isodon (Labiatae) have antitumor and antiinflammatory activities. We investigated the antiinflammatory effect of inflexinol in RAW 264.7 cells and astrocytes. As a result, we found that inflexinol (1, 5, 10 µM) suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells and astrocytes. Consistent with the inhibitory effect on iNOS and COX-2 expression, inflexinol also inhibited transcriptional and DNA binding activity of NF-κB via inhibition of IκB degradation as well as p50 and p65 translocation into nucleus. These results suggest that inflexinol inhibits iNOS and COX-2 expression through inhibition of NF-κB activation, thereby inhibits generation of inflammatory mediators in RAW 264.7 cells and astrocytes, and may be useful for treatment of inflammatory diseases.
- Subjects
DITERPENES; ANTI-inflammatory agents; NITRIC oxide; NF-kappa B; ASTROCYTES; CELLS; CYCLOOXYGENASE 2
- Publication
Mediators of Inflammation, 2007, Vol 2007, Issue 1, p1
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1155/2007/93148