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- Title
Presynaptic Ca<sub>v</sub>1.3 Channels Regulate Synaptic Ribbon Size and Are Required for Synaptic Maintenance in Sensory Hair Cells.
- Authors
Sheets, Lavinia; Kindt, Katie S.; Nicolson, Teresa
- Abstract
L-type calcium channels (Cav1) are involved in diverse processes, such as neurotransmission, hormone secretion, muscle contraction, and gene expression. In this study, we uncover a role for Cav 1.3a in regulating the architecture of a cellular structure, the ribbon synapse, in developing zebrafish sensory hair cells. By combining in vivo calcium imaging with confocal and super-resolution structured illumination microscopy, we found that genetic disruption or acute block of Cav1.3a channels led to enlargement of synaptic ribbons in hair cells. Conversely, activating channels reduced both synaptic-ribbon size and the number of intact synapses. Along with enlarged presynaptic ribbons in Cav1.3a mutants, we observed a profound loss of juxtaposition between presynaptic and postsynaptic components. These synaptic defects are not attributable to loss of neurotransmission, because vglut3 mutants lacking neurotransmitter release develop relatively normal hair-cell synapses. Moreover, regulation of synaptic-ribbon size by Ca2+ influx may be used by other cell types, because we observed similar pharmacological effects on pinealocyte synaptic ribbons. Our results indicate that Ca2+ influx through Cav1.3 fine tunes synaptic ribbon size during hair-cell maturation and that Cav1.3 is required for synaptic maintenance.
- Subjects
PRESYNAPTIC receptors; CALCIUM channels regulation; NEURAL transmission; HORMONE regulation; MUSCLE contraction; GENE expression; LABORATORY zebrafish
- Publication
Journal of Neuroscience, 2012, Vol 32, Issue 48, p17273
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.3005-12.2012