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- Title
Temporal characterisation of amphetamine-induced dopamine release assessed with [<sup>11</sup>C]raclopride in anaesthetised rodents.
- Authors
Gavin C. Houston; Susan P. Hume; Ella Hirani; Julian L. Goggi; Paul M. Grasby
- Abstract
Competition between endogenous neurotransmitters and radiolabelled tracers, as measured by positron emission tomography (PET), may provide a measure of endogenous neurotransmitter flux in vivo. For example, carbon-11 labelled raclopride has been effectively used to monitor dopamine release following pharmacological and behavioural manipulations. The current study describes a rodent model of amphetamine-induced [11C]raclopride reduction, which allowed the characterisation of the doseresponse and temporal dynamics of this reduction over a 24-h time course. Over the range studied, a monotonic doseresponse relationship between amphetamine dose and [11C]raclopride reduction was observed. When compared with previously published microdialysis data, an approximate 16% reduction in [11C]raclopride binding potential was associated with a ~25-fold increase in extracellular dopamine. A reduction of 2030% in raclopride binding was observed 30 min after amphetamine injection (4 mg/kg IP). This reduction in [11C]raclopride binding persisted for 4 h but returned to baseline by 8 h. The data suggest a persistent amphetamine-induced raclopride displacement in rodents and reinforce findings from nonhuman primates that a simple competitive occupancy model may not adequately explain the temporal characteristics of the amphetamine-induced decrease in radiotracer binding. Synapse 51:206212, 2004. © 2003 Wiley-Liss, Inc.
- Subjects
NEUROTRANSMITTERS; POSITRON emission; TOMOGRAPHY; DOPAMINE
- Publication
Synapse, 2004, Vol 51, Issue 3, p206
- ISSN
0887-4476
- Publication type
Article
- DOI
10.1002/syn.10296