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- Title
The Functions of Pt-DIC and Pt-Lamin B in Spermatogenesis of Portunus trituberculatus.
- Authors
Wang, Shuo-Yue; Xiang, Qiu-Meng; Zhu, Jun-Quan; Mu, Chang-Kao; Wang, Chun-Lin; Hou, Cong-Cong
- Abstract
Cytoplasmic Dynein is a multiple-subunit macromolecular motor protein involved in the transport process of cells. The Dynein intermediate chain (DIC) is one of the subunits of Dynein-1. In our previous studies, we showed that Pt-DIC may play an important role in the nuclear deformation of spermiogenesis in Portunus trituberculatus. Lamin B is essential for maintaining nuclear structure and functions. Surprisingly, Pt-Lamin B was expressed not only in the perinucleus but also in the pro-acrosome during spermiogenesis in P. trituberculatus. Studies have also shown that Dynein-1 can mediate the transport of Lamin B in mammals. Thus, to study the relationship of Pt-DIC and Pt-Lamin B in the spermatogenesis of P. trituberculatus, we knocked down the Pt-DIC gene in P. trituberculatus by RNAi. The results showed that the distribution of Pt-DIC and Pt-Lamin B in spermiogenesis was abnormal, and the colocalization was weakened. Moreover, we verified the interaction of Pt-DIC and Pt-Lamin B via coimmunoprecipitation. Therefore, our results suggested that both Pt-DIC and Pt-Lamin B were involved in the spermatogenesis of P. trituberculatus, and one of the functions of Dynein-1 is to mediate the transport of Lamin B in the spermiogenesis of P. trituberculatus.
- Subjects
PORTUNUS; NUCLEAR shapes; SPERMATOGENESIS; NUCLEAR structure; PROTEIN transport; CARRIER proteins; MOLECULAR motor proteins
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 1, p112
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25010112