We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
High-cholesterol diet enriched with onion affects endothelium-dependent relaxation in mesenteric microvessels and NADPH oxidase activity in Wistar rats.
- Authors
González-Peña, Diana; Angulo, Javier; Vallejo, Susana; Colina-Coca, Clara; de Ancos, Begoña; Sánchez-Ferrer, Carlos F.; Peiró, Concepción; Sánchez-Moreno, Concepción
- Abstract
Background: The aim of the present study was to examine the effects of onion as functional ingredient on the oxidative status, lipoprotein levels (total cholesterol-TC, HDL-C, LDL-C), triacylglycerides (TAG) and vascular reactivity of mesenteric arteries in hypercholesterolemic Wistar rats. Methods: Twenty-four animals were fed with three different diets [control, high-cholesterol diet (HC) and high-cholesterol enriched with onion diet (HCO)]. After seven weeks of experimental feeding the rats were euthanized for blood and tissues collection. TC, HDL-C, LDL-C and TAG were measured, and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+) scavenging capacity and ferric reducing antioxidant power (FRAP) were determined in plasma. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities were assayed in erythrocyte lysates. Endothelium-dependent vasodilation to acetylcholine was evaluated in mesenteric arterial segments. NADPH oxidase (NOX) was also measured by lucigenin-derived chemiluminiscence. Results: The dietary cholesterol content significantly affected plasma lipoprotein levels, increased superoxide generation from NOX, and caused impaired endothelium-dependent vasodilation in the rat mesenteric arteries. Onion ingredient improved antioxidant status in HCO group, as it was evidenced by ABTS+ and FRAP values and SOD and GPx enzyme activities compared to the HC-fed group, reduced the increment in NOX activity and reversed endothelial dysfunction promoted by the HC diet. Scavenging of superoxide with TEMPOL or inhibition of NOX with apocynin improved endothelium-dependent vasodilation only in HC-fed rats. Conclusions: Enrichment of diet with onion as functional ingredient could be proposed as a complementary approach to prevent or partially modulate vascular dysfunction, reducing some of the risk indices linked to initial development of atherosclerosis.
- Subjects
HYPERCHOLESTEREMIA prevention; ONIONS; ANIMAL experimentation; LIPIDS; LIPOPROTEINS; RATS; FUNCTIONAL foods; THERAPEUTICS
- Publication
Nutrition & Metabolism, 2014, Vol 11, Issue 1, p44
- ISSN
1743-7075
- Publication type
Article
- DOI
10.1186/1743-7075-11-57