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- Title
Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells
- Authors
Takano, Hirotsugu; Nakazawa, Shunsuke; Okuno, Yasushi; Shirata, Naritoshi; Tsuchiya, Sohken; Kainoh, Takayuki; Takamatsu, Seigoh; Furuta, Kazuyuki; Taketomi, Yoshitaka; Naito, Yuko; Takematsu, Hiromu; Kozutsumi, Yasunori; Tsujimoto, Gozoh; Murakami, Makoto; Kudo, Ichiro; Ichikawa, Atsushi; Nakayama, Kazuhisa; Sugimoto, Yukihiko; Tanaka, Satoshi
- Abstract
Abstract: To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.
- Subjects
MAST cells; FIBROBLASTS; GENE expression; CELL differentiation
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1444
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.033