We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Afaf, a novel vesicle membrane protein, is related to acrosome formation in murine testis
- Authors
Li, Yin-Chuan; Hu, Xiao-Qian; Zhang, Ke-Ying; Guo, Jian; Hu, Zhao-Yuan; Tao, Shi-Xin; Xiao, Li-Juan; Wang, Qing-Zhong; Han, Chun-Sheng; Liu, Yi-Xun
- Abstract
Abstract: As a cell-specific organelle, acrosome (Acr) and its formation are an important event for spermiogenesis. However, the Acr formation is far more complicated than has been proposed. In this study, we have cloned a novel membrane protein Afaf (Acr formation associated factor) that was expressed abundantly in the round spermatids, localized in the inner and outer membrane of forming Acrs, and declined in the maturing Acrs. In the transfected Hela cells, Afaf protein was localized in the plasma membrane, EEA1-positive early endosomes (EEs) and occasionally in the nuclei. Therefore, we propose that EEs and plasma membrane may be also directly involved in the Acr biogenesis.
- Subjects
ACROSOME reaction; SPERMIOGENESIS in animals; MEMBRANE proteins; CANCER cells
- Publication
FEBS Letters, 2006, Vol 580, Issue 17, p4266
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2006.06.010