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- Title
FISH and HER2/ equivocal immunohistochemistry in breast carcinoma.
- Authors
Patil Okaly, Geeta; Panwar, Dipti; Lingappa, Kavitha; Kumari, Prasanna; Anand, Abhishek; Kumar, Prashantha; Chikkalingaiah, Manju; Kumar, Rekha; Patil Okaly, Geeta Vikram; Lingappa, Kavitha Bidadli; Chikkalingaiah, Manju Hosur; Kumar, Rekha Vijay
- Abstract
<bold>Aim: </bold>The aim of this study was to validate the role of fluorescence in situ hybridization (FISH) in investigating HER2/neu gene amplification (human epidermal growth factor receptor 2) in patients with HER2/neu equivocal breast cancer diagnosed on immunohistochemistry (IHC).<bold>Materials and Methods: </bold>This was a retrospective study conducted from January 2013 to October 2017. A total of 134 patients diagnosed with invasive breast carcinoma and HER2/neu equivocal status on IHC were analyzed. Also, the cases for the years 2016 and 2017 formed a subgroup that was analyzed further to study the impact of pre-analytical factors on IHC and FISH results.<bold>Results: </bold>A total of 134 women with HER2/neu IHC equivocal breast cancer were included in the study with a median age of 50 years (range 25-81). HER2/neu amplification by FISH was noted in 72 (54%) cases, whereas it was non-amplified in 52 (39%) cases. Ten cases were reported as equivocal even on FISH (ASCO/CAP 2013 guidelines). Polysomy 17 was noted in 55 cases (41%), of which 26 patients were≤50 years and 29 patients were >50 years of age. Twenty (36%) of these 55 cases showed HER2/neu amplification, whereas 26 (48%) cases were non-amplified and 9 (16%) cases were reported as equivocal on FISH. Also, more than half of the polysomy cases were hormone receptor negative.<bold>Conclusion: </bold>IHC is a good screening tool for negative and positive results. Any patient targeted for trastuzumab therapy should undergo confirmation of HER2/neu equivocal status by FISH analysis. We also suggest that if a non-classical FISH pattern is seen, the test should be repeated with a non-centromeric chromosome 17 reference locus probe for better treatment planning.
- Subjects
HORMONE receptor positive breast cancer; EPIDERMAL growth factor receptors; FLUORESCENCE in situ hybridization; FISHES
- Publication
Indian Journal of Cancer, 2019, Vol 56, Issue 2, p119
- ISSN
0019-509X
- Publication type
journal article
- DOI
10.4103/ijc.IJC_333_18