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- Title
Glycerol Dehydratases: Biochemical Structures, Catalytic Mechanisms, and Industrial Applications in 1,3-Propanediol Production by Naturally Occurring and Genetically Engineered Bacterial Strains.
- Authors
Liu, Jian-zhong; Xu, Wu; Chistoserdov, Andrei; Bajpai, Rakesh
- Abstract
To date, two types of glycerol dehydratases have been reported: coenzyme B-dependent and coenzyme B-independent glycerol dehydratases. The three-dimensional structure of the former is a dimer of αβγ heterotrimer, while that of the latter is a homodimer. Their mechanisms of reaction are typically enzymatic radical catalysis. Functional radical in both the glycerol dehydratases is the adenosyl radical. However, the adenosyl radical in the former originates from coenzyme B by homolytic cleavage, and that in the latter from S-adenosyl-methionine. Until some years ago, Clostridium butyricum VPI 1718 was the only microorganism known to possess B-independent glycerol dehydratase, but since then, several other bacteria with this unique capability have been identified. This article focuses on the glycerol dehydratases and on 1,3-propanediol production from glycerol by naturally occurring and genetically engineered bacterial strains containing glycerol dehydratase.
- Subjects
GLYCERIN; PROPYLENE glycols; COENZYMES; CLOSTRIDIUM butyricum; X-ray crystallography
- Publication
Applied Biochemistry & Biotechnology, 2016, Vol 179, Issue 6, p1073
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-016-2051-6