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- Title
A Leucine-Rich Repeat Receptor-like Kinase from the Antarctic Moss Pohlia nutans Confers Salinity and ABA Stress Tolerance.
- Authors
Wang, Jing; Zhang, Pengying; Liu, Shenghao; Cong, Bailin; Chen, Kaoshan
- Abstract
Plant leucine-rich repeats receptor-like kinases (LRR-RLKs) play key roles in plant growth, development, and responses to environmental stresses. However, the functions of LRR-RLKs in bryophytes are still not well documented. Here, a putative LRR-RLK gene, PnLRR-RLK, was cloned and characterized from the Antarctic moss Pohlia nutans. Phylogenetic analysis revealed that PnLRR-RLK protein was clustered with the Arabidopsis thaliana LRR XI family proteins. Subcellular localization analysis of PnLRR-RLK revealed that it was mainly localized on plasma membrane. The expression of PnLRR-RLK was induced by mock high salinity, cold, drought, and exogenously supplied abscisic acid (ABA) and methyl jasmonate (MeJA). Meanwhile, the overexpression of PnLRR-RLK showed an increased tolerance of transgenic Arabidopsis to salt and ABA stresses than that of the wild type (WT) plants. Furthermore, the expression levels of several salt tolerance genes ( AtHKT1, AtSOS3, AtP5CS1, and AtADH1) and an ABA negatively regulating gene AtABI1 were significantly increased in transgenic plants. Meanwhile, the expression levels of ABA biosynthesis genes ( AtNCED3, AtABA1, and AtAAO3) and ABA early response genes ( AtMYB2, AtRD22, AtRD29A, and AtDREB2A) were decreased in transgenic Arabidopsis after salt stress treatment. Therefore, these results suggested that PnLRR-RLK might involve in regulating salt stress-related and ABA-dependent signaling pathway, thereby contribute to the salinity tolerance of the Antarctic moss P. nutans.
- Subjects
RECEPTOR-like kinases; PLANT phylogeny; DARDARIN; PLANT proteins; ARABIDOPSIS thaliana; GENETIC overexpression; EFFECT of stress on plants; ENVIRONMENTAL engineering
- Publication
Plant Molecular Biology Reporter, 2016, Vol 34, Issue 6, p1136
- ISSN
0735-9640
- Publication type
Article
- DOI
10.1007/s11105-016-0994-y