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- Title
3-(2-Bromoacetamido)- N-(9-ethyl-9 H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.
- Authors
You, Jinmao; Dou, Kun; Song, Cuihua; Li, Guoliang; Sun, Zhiwei; Zhang, Shijuan; Chen, Guang; Zhao, Xianen; Hu, Na; Zhou, Wu
- Abstract
A rapid, sensitive, and selective precolumn derivatization method for the simultaneous determination of eight thiophenols using 3-(2-bromoacetamido)- N-(9-ethyl-9 H)-carbazol as a labeling reagent by high-performance liquid chromatography with fluorescence detection has been developed. The labeling reagent reacted with thiophenols at 50°C for 50 min in aqueous acetonitrile in the presence of borate buffer (0.10 mol/L, pH 11.2) to give high yields of thiophenol derivatives. The derivatives were identified by online postcolumn mass spectrometry. The collision-induced dissociation spectra for thiophenol derivatives gave the corresponding specific fragment ions at m/z 251.3, 223.3, 210.9, 195.8, and 181.9. At the same time, derivatives exhibited intense fluorescence with an excitation maximum at λex = 276 nm and an emission maximum at λem = 385 nm. Excellent linear responses were observed for all analytes over the range of 0.033-6.66 μmol/L with correlation coefficients of more than 0.9997. Detection limits were in the range of 0.94-5.77 μg/L with relative standard deviations of less than 4.54%. The feasibility of derivatization allowed the development of a rapid and highly sensitive method for the quantitative analysis of trace levels of thiophenols from some rubber products. The average recoveries ( n = 3) were in the range of 87.21-101.12%.
- Subjects
THIOPHENOL; CARBAZOLE; LIQUID chromatography; FLUORESCENCE spectroscopy; RUBBER goods
- Publication
Journal of Separation Science, 2017, Vol 40, Issue 12, p2528
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201601166