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- Title
Biosynthesis of Chuangxinmycin Featuring a Deubiquitinase‐like Sulfurtransferase.
- Authors
Zhang, Xingwang; Xu, Xiaokun; You, Cai; Yang, Chaofan; Guo, Jiawei; Sang, Moli; Geng, Ce; Cheng, Fangyuan; Du, Lei; Shen, Yuemao; Wang, Sheng; Lan, Haidong; Yang, Fan; Li, Yuezhong; Tang, Ya‐Jie; Zhang, Youming; Bian, Xiaoying; Li, Shengying; Zhang, Wei
- Abstract
The knowledge on sulfur incorporation mechanism involved in sulfur‐containing molecule biosynthesis remains limited. Chuangxinmycin is a sulfur‐containing antibiotic with a unique thiopyrano[4,3,2‐cd]indole (TPI) skeleton and selective inhibitory activity against bacterial tryptophanyl‐tRNA synthetase. Despite the previously reported biosynthetic gene clusters and the recent functional characterization of a P450 enzyme responsible for C−S bond formation, the enzymatic mechanism for sulfur incorporation remains unknown. Here, we resolve this central biosynthetic problem by in vitro biochemical characterization of the key enzymes and reconstitute the TPI skeleton in a one‐pot enzymatic reaction. We reveal that the JAMM/MPN+ protein Cxm3 functions as a deubiquitinase‐like sulfurtransferase to catalyze a non‐classical sulfur‐transfer reaction by interacting with the ubiquitin‐like sulfur carrier protein Cxm4GG. This finding adds a new mechanism for sulfurtransferase in nature.
- Subjects
BIOSYNTHESIS; CARRIER proteins; GENE clusters; SULFUR metabolism; SULFUR
- Publication
Angewandte Chemie, 2021, Vol 133, Issue 46, p24623
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202107745