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- Title
Electrochemical Quantification of Escherichia coli with DNA Nanostructure.
- Authors
Giovanni, Marcella; Setyawati, Magdiel Inggrid; Tay, Chor Yong; Qian, Hang; Kuan, Win Sen; Leong, David Tai
- Abstract
Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL -1 E. coli lipopolysaccharides and 1.20 CFU mL-1 equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.
- Subjects
DNA; ESCHERICHIA coli; FOOD poisoning; LIPOPOLYSACCHARIDES; MACROMOLECULES
- Publication
Advanced Functional Materials, 2015, Vol 25, Issue 25, p3840
- ISSN
1616-301X
- Publication type
Article
- DOI
10.1002/adfm.201500940