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- Title
Conjugated Polyelectrolyte and Aptamer Based Potassium Assay via Single- and Two-Step Fluorescence Energy Transfer with a Tunable Dynamic Detection Range.
- Authors
Nguyen, Bao Lam; Jeong, Ji‐Eun; Jung, In Hwan; Kim, Boram; Le, Van Sang; Kim, Inhong; Kyhm, Kwangseuk; Woo, Han Young
- Abstract
A new potassium ion detection assay was developed using a dye-labeled aptamer and conjugated polyelectrolyte (CPE) as a signaling platform via 1-step and 2-step fluorescence resonance energy transfer. Guanine-rich K+-specific aptamers were designed as K+ ion recognition species with 6-carboxyfluorescein (6-FAM) and 6-carboxytetramethylrhodamine (6-TAMRA) at both termini. In the presence of K+ ions, the aptamers undergo a conformational change from an unfolded to folded form by forming a G-quadruplex with K+, bringing two dyes in proximity. FRET-induced 6-TAMRA emission was proportional to [K+] in a range of 22.5 μ m-100 m m in water without interference by the presence of excess Na+ ions (100 m m). Upon the addition of CPE, a two-step FRET process from CPE to 6-TAMRA via 6-FAM was enabled, showing an intensified 6-TAMRA signal with K+ ions. The dynamic detection range and limit of detection (LOD) was fine-tuned from ∼millimolar to ∼nanomolar concentrations of K+ by modulating the signal amplification effect of CPE. The LOD was determined to be ≈3.0 n m. This detection assay also showed high selectivity against other metal ions. This sensing scheme can be extended to the detection of a wide range of target materials by simply modifying the recognition aptamer sequence.
- Subjects
CONDUCTIVITY of polyelectrolytes; APTAMERS; FIRE assay; FLUORESCENCE resonance energy transfer; AMPLIFICATION reactions; CONFORMATIONAL analysis
- Publication
Advanced Functional Materials, 2014, Vol 24, Issue 12, p1748
- ISSN
1616-301X
- Publication type
Article
- DOI
10.1002/adfm.201301515