We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The role of miR-433-3p in vascular calcification in type 2 diabetic patients: targeting WNT/β-Catenin and RANKL/RANK/OPG signaling pathways.
- Authors
Elshamy, Amira M.; Hafez, Yasser Mostafa; Safa, Mohamed A. E.; Ibrahim, Hoda A.; Khalfallah, Mohamed; Rizk, Fatma H.; Eltabaa, Eman F.; Ghafar, Muhammad T. Abdel; Atef, Marwa Mohamed
- Abstract
Background: Vascular calcification (VC) is a major predictor of cardiovascular diseases that represent the principal cause of mortality among type-2 diabetic patients. Accumulating data suggest the vital role of some microRNAs on vascular calcification as an epigenetic regulator. Thus, we assessed herein, the role of serum miR-433-3p in vascular calcification in type-2 diabetic patients. Methods: Twenty healthy subjects (control group) and forty diabetic patients (20 without VC and 20 with VC) were involved in the study. miR-433-3p gene expression was measured. Runx2, Dickkopf-1 (DKK1), β-catenin, Receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) levels in serum were assessed by ELISA technique. Results: Diabetes patients had significantly lower levels of miR-433-3p expression in comparison to the control group, with the lowest levels being found in diabetic patients with VC. Furthermore, Runx2, β-catenin, and RANKL levels were significantly increased with concomitant lower DKK1 and OPG levels detected in the two diabetic groups especially those with VC. Conclusion: Collectively, the study documented that down-regulation of miR-433-3p may contribute to the development of VC through activating WNT/β-Catenin and RANKL/RANK/OPG signaling pathways.
- Subjects
WNT signal transduction; CATENINS; ARTERIAL calcification; TRANCE protein; CALCIFICATION; HYPERGLYCEMIA; PEOPLE with diabetes; CELLULAR signal transduction
- Publication
Molecular Biology Reports, 2023, Vol 50, Issue 11, p9073
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-023-08792-9