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- Title
Spontaneous recombinase activity of Cre-ERT2 in vivo.
- Authors
Kristianto, Jasmin; Johnson, Michael; Zastrow, Ryley; Radcliff, Abigail; Blank, Robert
- Abstract
Inducible Cre-ERT recombinase technology is widely used for gene targeting studies. The second generation of inducible Cre-ERT recombinase, hemizygous B6.129S-Tg(UBC-cre/ERT2)1Ejb/J (hereafter abbreviated as Cre- ERT2), a fusion of a mutated estrogen receptor and Cre recombinase, was engineered to be more efficient and specific than the original Cre-ERT. The putative mechanism of selective Cre-mediated recombination is Cre sequestration in the cytoplasm in the basal state with translocation to the nucleus only in the presence of tamoxifen. We utilized both a reporter mouse (B6.129 (Cg)- Gt( ROSA) 26Sor /J) and endothelin converting enzyme-1 floxed transgenic mouse line to evaluate Cre-ERT2 activity. We observed spontaneous Cre activity in both settings. Unintended Cre activity is a confounding factor that has a potentially large impact on data interpretation. Thus, it is important to consider background Cre activity in experimental design.
- Subjects
RECOMBINASES; ESTROGEN receptors; CYTOPLASM; TAMOXIFEN; SEQUESTRATION (Chemistry)
- Publication
Transgenic Research, 2017, Vol 26, Issue 3, p411
- ISSN
0962-8819
- Publication type
Article
- DOI
10.1007/s11248-017-0018-1