We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
S-Nitrosoglutathione ameliorates acute renal dysfunction in a rat model of lipopolysaccharide-induced sepsis.
- Authors
Samuvel, Devadoss J.; Shunmugavel, Anandakumar; Singh, Avtar K.; Singh, Inderjit; Khan, Mushfiquddin
- Abstract
Objective Sepsis induces an inflammatory response that results in acute renal failure ( ARF). The current study is to evaluate the role of S-Nitrosoglutathione ( GSNO) in renoprotection from lipopolysaccharide ( LPS)-induced sepsis. Methods Rats were divided to three groups. First group received LPS (5 mg/kg body weight), second group was treated with LPS + GSNO (50 μg/kg body weight), and third group was administered with vehicle (saline). They were sacrificed on day 1 and 3 post- LPS injection. Serum levels of nitric oxide ( NO), creatinine and blood urea nitrogen ( BUN) were analysed. Tissue morphology, T lymphocyte infiltrations, and the expression of inflammatory ( TNF-α, i NOS) and anti-inflammatory ( IL-10) mediators as well as glutathione ( GSH) levels were determined. Key finding Lipopolysaccharide significantly decreased body weight and increased cellular T lymphocyte infiltration, caspase-3 and i NOS and decreased PPAR-γ in renal tissue. NO, creatinine and BUN were significantly elevated after LPS challenge, and they significantly decreased after GSNO treatment. TNF-α level was found significantly increased in LPS-treated serum and kidney. GSNO treatment of LPS-challenged rats decreased caspase-3, iNOS, TNF-α, T lymphocyte infiltration and remarkably increased levels of IL-10, PPAR-γ and GSH. Conclusion GSNO can be used as a renoprotective agent for the treatment of sepsis-induced acute kidney injury.
- Subjects
S-nitrosoglutathione; S-Nitrosothiols; SEPSIS; LABORATORY rats; NITROSO compounds
- Publication
Journal of Pharmacy & Pharmacology, 2016, Vol 68, Issue 10, p1310
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12608