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- Title
Evaluation of butyrate-induced production of a mannose-6-phosphorylated therapeutic enzyme using parallel bioreactors.
- Authors
Madhavarao, Chikkathur N.; Agarabi, Cyrus D.; Wong, Lily; Müller‐Loennies, Sven; Braulke, Thomas; Khan, Mansoor; Anderson, Howard; Johnson, Gibbes R.
- Abstract
Bioreactor process changes can have a profound effect on the yield and quality of biotechnology products. Mannose-6-phosphate (M6P) glycan content and the enzymatic catalytic kinetic parameters are critical quality attributes ( CQAs) of many therapeutic enzymes used to treat lysosomal storage diseases ( LSDs). Here, we have evaluated the effect of adding butyrate to bioreactor production cultures of human recombinant
- Subjects
MANNOSE 6-phosphate; LYSOSOMAL storage diseases; THERAPEUTICS; BIOREACTORS; THERAPEUTIC use of enzymes; GLYCANS; CAPILLARY electrophoresis
- Publication
Biotechnology & Applied Biochemistry, 2014, Vol 61, Issue 2, p184
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1151