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- Title
Turgor Pressure Regulation and the Orientation of Cortical Microtubules in Spirogyra Cells.
- Authors
Iwata, Kazuyoshi; Tazawa, Masashi; Itoh, Takao
- Abstract
Microtubules (MTs) of cells of Spirogyra sp. were depolymerized by treatment with amiprophos-methyl (APM) for 1 h and then reorganized in 0.30 M mannitol solution. The reorganized MTs after 1.5 h incubationshowed an oblique/longitudinal orientation and then became transversely oriented as the incubation was prolonged. During this incubation, the osmotic pressure of cells was measured by the plasmolysis method. The cell osmotic pressure increased with time.The calculatedturgor pressure at 1.5 h was 0.11 M (mannitol equivalent) and, at 13.5 h, 0.25 M. Similar changes in MT orientation and recovery of the turgor pressure were also observed in 0.30 M sorbitol solution. These results suggest that the MT orientation may be correlated with the turgor pressure.
- Subjects
SPIROGYRA; TURGOR; MICROTUBULES; PLANT cells &; tissues; OSMOSIS
- Publication
Plant & Cell Physiology, 2001, Vol 42, Issue 6, p594
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pce073