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- Title
Bioluminescence Imaging of Matrix Metalloproteinases-2 and -9 Activities in Ethanol-injured Cornea of Mice.
- Authors
SEUNG-BO PARK; SEUNG HYO JUNG; HENGZHE JIN; SU JUNG KIM; YUNKYOUNG RYU; KYUNG-JIN LEE; BOKYUNG KIM; HYUN JIN SHIN; KYUNG-JONG WON
- Abstract
Background/Aim: This study aimed to investigate the usefulness of in vivo bioluminescence imaging (BLI) to examine the role of matrix metalloproteinases (MMP)-2 and MMP-9 activation in the development and healing of ethanol-induced damage in the cornea of mice. Materials and Methods: Mouse corneal injury was induced by topical treatment with 20% ethanol. BLI was obtained from the ocular region of mice intravenously injected with an active-MMP-2/9 probe. In vivo results were validated in primary corneal epithelial cells. Results: BLI indicated that treatment of the eye with 20% ethanol elevated MMP-2/9 activity, which was inhibited by the application of eye drops (hyaluronic acid and serum). Treatment of corneal epithelial cells with 20% ethanol-increased the activities of MMP-2 and MMP-9, which were also inhibited by eye drops. Conclusion: BLI can be applied in vivo in mice with corneal injury to examine the activity of MMPs and clarify the efficacy of eye drops.
- Subjects
MATRIX metalloproteinases; BIOLUMINESCENCE; CORNEA injuries; ETHANOL; LABORATORY animals
- Publication
In Vivo, 2021, Vol 35, Issue 3, p1521
- ISSN
0258-851X
- Publication type
Article
- DOI
10.21873/invivo.12405