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- Title
Decellularized Wharton's Jelly and Amniotic Membrane Demonstrate Potential Therapeutic Implants in Tracheal Defects in Rabbits.
- Authors
Neto, Aloysio Enck; Foltz, Katia Martins; Fuchs, Thiago; Gamba, Luize Kremer; Denk, Marcos Antonio; Silveira, Paulo Cesar Lock; Nascimento, Thatyanne Gradowski do; Clemencia, Alice Machado; Francisco, Julio César; Noronha, Lucia de; Guarita-Souza, Luiz César
- Abstract
Background: Tracheal grafts have been investigated for over a century, aiming to replace various lesions. However, tracheal reconstruction surgery remains a challenge, primarily due to anatomical considerations, intraoperative airway management, the technical complexity of reconstruction, and the potential postoperative morbidity and mortality. Due to research development, the amniotic membrane (AM) and Wharton's Jelly (WJ) arise as alternatives within the new set of therapeutic alternatives. These structures hold significant therapeutic potential for tracheal defects. This study analyzed the capacity of tracheal tissue regeneration after 60 days of decellularized WJ and AM implantation in rabbits submitted to conventional tracheostomy. Methods: An in vivo experimental study was carried out using thirty rabbits separated into three groups (Control, AM, and WJ) (n = 10). The analyses were performed 60 days after surgery through immunohistochemistry. Results: Different immunomarkers related to scar regeneration, such as aggrecan, TGF-β1, and α-SMA, were analyzed. However, they highlighted no significant difference between the groups. Collagen type I, III, and Aggrecan also showed no significant difference between the groups. Conclusions: Both scaffolds appeared to be excellent frameworks for tissue engineering, presenting biocompatibility and a desirable microenvironment for cell survival; however, they did not display histopathological benefits in trachea tissue regeneration.
- Subjects
AMNION; RABBITS; BONE regeneration; JELLY; TISSUE engineering; CELL survival
- Publication
Life (2075-1729), 2024, Vol 14, Issue 6, p782
- ISSN
2075-1729
- Publication type
Article
- DOI
10.3390/life14060782