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- Title
Antioxidant and Cytotoxic Activities of the Essential Oil and Extracts of Anisosciadium orientale.
- Authors
Masroorbabanari, Mehdi; Miri, Ramin; Firuzi, Omidreza; Jassbi, Amir Reza
- Abstract
The essential oil of Anisosciadium orientale was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The essential oil (EO) was rich in monoterpene hydrocarbons: terpinolene (19.7±2.8%, 226.4±27.6 mg/ g EO), limonene (21.0±1.3%, 313.3±22.9 mg/ g EO) and myrcene (5.6±0.50 %, 65.4±7.2 mg/ g EO); and the phenylpropanoid myristicin (30.6±2.3%, 438.5±40.0 mg/ g EO). The sesquiterpene germacrene D constituted 6.1±0.8%, 91.8±10.6 mg/ g EO of the oil. The essential oil and the plant's methanol and 80% methanol extracts were screened for antioxidant activity and total phenol content using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and Folin-Ciocalteu assays, respectively. The radical scavenging activity (lower IC50s; μg EO or the plant material (PM) /ml 10-4 M DPPH solution) was positively correlated to total phenolic content expressed as mg equivalents of gallic acid (EG) in 1 g EO or in the PM (IC50 633.2±0.2 μg EO /ml DPPH; 8.5±0.2 mg EG/g EO), the methanol extracts (IC50 400.0±0.2 μg PM /ml DPPH; 10.3±0.5 mg EG/g PM), and 80% methanol extracts (IC50 220.0±0.0 μg PM /ml DPPH; 15.9±0.2 mg EG/g PM), respectively. The cytotoxic activity of the essential oil was measured on 3 human cancer cell lines including K562 (human chronic myelogenous leukemia), LS180 (human colon adenocarcinoma) and MCF-7 (human breast adenocarcinoma) cells. The IC50 values of the oil on K562, LS180 and MCF-7 cells were 396.8 ± 64.9, 183.2 ± 42.8 and 159.5 ± 29.0 μg/ml, respectively (mean ± SE), while the plant extracts were not active.
- Subjects
ANTIOXIDANTS; ESSENTIAL oils; GAS chromatography/Mass spectrometry (GC-MS); HYDROCARBONS; FLAME ionization detectors; BREAST cancer treatment
- Publication
Journal of the Chemical Society of Pakistan, 2014, Vol 36, Issue 3, p457
- ISSN
0253-5106
- Publication type
Article