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- Title
Yersinia pseudotuberculosis BarA-UvrY Two-Component Regulatory System Represses Biofilms via CsrB.
- Authors
Schachterle, Jeffrey K.; Stewart, Ryan M.; Schachterle, M. Brett; Calder, Joshua T.; Huan Kang; Prince, John T.; Erickson, David L.
- Abstract
The formation of biofilms by Yersinia pseudotuberculosis (Yptb) and Y. pestis requires he hmsHFRS genes, which direct production of a polysaccharide extracellular matrix Hms-ECM). Despite possessing identical hmsHFRS sequences, Yptb produces much ess Hms-ECM than Y. pestis. The regulatory influences that control Yptb Hms-ECM production and biofilm formation are not fully understood. In this study, negative egulators of biofilm production in Yptb were identified. Inactivation of the BarA/UvrY wo-component system or the CsrB regulatory RNA increased binding of Congo Red dye, which correlates with extracellular polysaccharide production. These mutants also produced biofilms that were substantially more cohesive than the wild type strain. Disruption of uvrY was not sufficient for Yptb to cause proventricular blockage during nfection of Xenopsylla cheopis fleas. However, this strain was less acutely toxic toward eas than wild type Yptb. Flow cytometry measurements of lectin binding indicated hat Yptb BarA/UvrY/CsrB mutants may produce higher levels of other carbohydrates in addition to poly-GlcNAc Hms-ECM. In an effort to characterize the relevant downstream argets of the BarA/UvrY system, we conducted a proteomic analysis to identify proteins with lower abundance in the csrB::Tn5 mutant strain. Urease subunit proteins were less abundant and urease enzymatic activity was lower, which likely reduced toxicity toward eas. Loss of CsrB impacted expression of several potential regulatory proteins that may nfluence biofilms, including the RcsB regulator. Overexpression of CsrB did not alter the Congo-red binding phenotype of an rcsB::Tn5 mutant, suggesting that the effect of CsrB on biofilmsmay require RcsB. These results underscore the regulatory and compositional differences between Yptb and Y. pestis biofilms. By activating CsrB expression, the Yptb BarA/UvrY two-component system has pleiotropic effects that impact biofilm production and stability.
- Subjects
PSEUDOTUBERCULOSIS; BIOFILMS; GENE expression; YERSINIA pestis; MULTIDRUG resistance
- Publication
Frontiers in Cellular & Infection Microbiology, 2018, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2018.00323