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- Title
Uptake and accumulation of B-group vitamers in Saccharomyces cerevisiae in ethanol-stat fed-batch culture.
- Authors
Paalme, T.; Kevvai, K.; Vilbaste, A.; Hälvin, K.; Nisamedtinov, I.
- Abstract
The uptake and accumulation of the B-group vitamins thiamine, riboflavin, nicotinamide, pantothenic acid and pyridoxine in Saccharomyces cerevisiae was studied by gradually increasing the specific dosage of vitamins in an ethanol-stat fed-batch culture. Thiamine, nicotinamide, pantothenic acid, and pyridoxine were almost completely taken up at low vitamin dosages. Thiamine was determined to be the major accumulating form of vitamin B while most of the assimilated nicotinamide and pantothenic acid accumulated in cofactor forms. Despite the obvious uptake of pyridoxine, accumulation of B vitamers was not observed. In contrast with the other vitamins studied, riboflavin began accumulating in the culture medium immediately after vitamin addition was initiated. By the end of the experiment, the apparent uptake of all vitamins exceeded their accumulation in the cells. Variations in the growth rate of yeast at different vitamin dosages demonstrate the importance of balancing the vitamins in the media during cultivation.
- Subjects
SACCHAROMYCES cerevisiae; VITAMIN B1; VITAMIN B2; NICOTINAMIDE; PANTOTHENIC acid; VITAMIN B6
- Publication
World Journal of Microbiology & Biotechnology, 2014, Vol 30, Issue 9, p2351
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-014-1660-x