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- Title
Effects of bone morphogenetic protein-2 on proliferation of tongue cancer Tca8113.
- Authors
Jiang Juan; Zhang Jun; Wang Xuxia; Xue Liwei; Song Hongning; Zhang Xi
- Abstract
Objective: To investigate the morphology and proliferation effects of adenovirus containing bone morpho-genetic protein-2 (BMP-2) on tongue cancer Tca8113 cells. Methods: Tca8113 cells were transfected with the Ad-BMP-2 of 0, 50, 100 multiplicity of infection (MOD respectively. Inverted fluorescence microscope was used to evaluate the morphological changes of these cells. Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells. Methyl thiazolyl tetrazolium (MTT) assay was performed to monitor the proliferative activity of the infected Tca8113 cells and then the growth curve was made. Results: The transfection efficiency rea-ched the highest when the MOI was 100. Moreover, the expression of BMP-2 was detected in Tca8113 cells by Western blot. There were no obvious morphological changes of the Tca8113 cells before and after transfection. And the prolifera-tion of transfected Tca8113 cells decreased compared with control. Conclusion: Ad-BMP-2 gene can inhibit the pro-liferation of Tca8113 cells in vitro.
- Publication
West China Journal of Stomatology, 2011, Vol 29, Issue 5, p546
- ISSN
1000-1182
- Publication type
Article
- DOI
10.3969/j.issn.1000-1182.2011.05.025