We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
An experimental study of expression changes of inducible nitric oxide synthase in rat dental pulp during direct pulp capping with two capping agents.
- Authors
Zhang Xiaofang; Lin Yanju; Zhao Yu
- Abstract
Objective To observe the expression of inducible nitric oxide synthase (iNOS) in rat dental pulp after direct pulp capping with calcium hydroxide(CH) and mineral trioxide aggregate (MTA). Methods Samples from 42 Wistar rats were immediately collected at 1, 3, 7, 14, 21 and 28 days after direct pulp capping with CH and MTA. Pulp tissues were studided with immunohistochemical stain. Software for image analysis was used to test the optical density of each specimen. The data were analyzed by paired t-test and Dunnett-i test with SPSS 11.5. Results In normal pulp, the stain of iNOS was negative. After direct pulp capping with CH or MTA, iNOS expression demonstrated weakly positive, positive, weakly positive, negative at different time, the expression was low immediately after capping but became strong after 3 days. Until 7 days the stain became less positive gradually and at 21 days the stain approached normal. The expression was mainly at the fibroblasts, odontoblasts, vascular endothelial cells, neutrophils, etc near the bottom of the pulp capping agent. The diffence of iNOS expression was significant 3 days after direct pulp capping with this two capping agents. Conclusion After direct pulp capping, iNOS expression increasd at first and then decreased, the diffence of capping agents will have an impact on the expression of iNOS 3 days after direct pulp capping.
- Publication
West China Journal of Stomatology, 2011, Vol 29, Issue 4, p420
- ISSN
1000-1182
- Publication type
Article
- DOI
10.3969/j.issn.1000-1182.2011.04.022