The article presents information on the molecular biology techniques used to identify a unkonwn frog. DNA extraction was done, using a Qiagen DNEasy Blood and Tissue kit and mitochondrial DNA 16S rRNA gene was targeted. Nucleotide BLAST search (BLASTn), a phylogenetic search tool, was done for the unknown 16S gene on Genbank. The nucleotide similarity search matched with frogs of Lophiohylini and Cuban Treefrog family.