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- Title
一株烟草青枯拮抗细菌中活性物质 稳定性和粗提物成分分析.
- Authors
吴翔; 谢丽源; 甘炳成; 陈影; 彭卫红; 谭昊; 黄忠乾
- Abstract
[Objective] Bacteria MT-002-B-7 had good effect on the antagonism of tobacco bacterial wilt, the characteristics and the main components of its active antagonistic substances were studied. [Method] The characteristics of its active antagonistic substances was analyzed by the single factor condition analysis, and the main components of active antagonistic substances was analyzed by IR spectra and GC-MS. [Result] The result showed that the polarity of active antagonistic substances was similar to ethyl acetate. The active antagonistic substances had a good thermal stability, resistant to proteinase K, pepsin and trypsin. The activity of antagonistic substances were not affected by the storage time at normal temperature. But more than 8 hours of irradiating under 8 w ultraviolet radiation, pH <5 or pH >8 significantly reduced the activity. Hie results of IB spectra and GC-MS showed that the main components of active antagonistic substances were N-acetyl-3-methyl-1,4-two azabicyclo[4.3.0] -nonane-2,5-two ketone, fatty acids, and azophenvlene.
- Subjects
FATTY acids; THERMAL stability; FACTOR analysis; KETONES; ANTIBIOSIS; TRYPSIN; PROTEINASES; ETHYL acetate
- Publication
Southwest China Journal of Agricultural Sciences, 2019, Vol 32, Issue 7, p1549
- ISSN
1001-4829
- Publication type
Article
- DOI
10.16213/j.icnki.scjas.2019.7.015