We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
PINK1 is recruited to mitochondria with parkin and associates with LC3 in mitophagy
- Authors
Kawajiri, Sumihiro; Saiki, Shinji; Sato, Shigeto; Sato, Fumiaki; Hatano, Taku; Eguchi, Hiroto; Hattori, Nobutaka
- Abstract
Abstract: Mutations in PTEN-induced putative kinase 1 (PINK1) cause recessive form of Parkinson’s disease (PD). PINK1 acts upstream of parkin, regulating mitochondrial integrity and functions. Here, we show that PINK1 in combination with parkin results in the perinuclear mitochondrial aggregation followed by their elimination. This elimination is reduced in cells expressing PINK1 mutants with wild-type parkin. Although wild-type PINK1 localizes in aggregated mitochondria, PINK1 mutants localization remains diffuse and mitochondrial elimination is not observed. This phenomenon is not observed in autophagy-deficient cells. These results suggest that mitophagy controlled by the PINK1/parkin pathway might be associated with PD pathogenesis. Structured summary: MINT-7557195: PINK1 (uniprotkb:Q9BXM7) physically interacts (MI:0915) with LC3 (uniprotkb:Q9GZQ8) by anti tag coimmunoprecipitation (MI:0007) MINT-7557109: LC3 (uniprotkb:Q9GZQ8) and PINK1 (uniprotkb:Q9BXM7) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7557121: tom20 (uniprotkb:Q15388) and PINK1 (uniprotkb:Q9BXM7) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7557138: parkin (uniprotkb:O60260), PINK1 (uniprotkb:Q9BXM7) and tom20 (uniprotkb:Q15388) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7557173: LC3 (uniprotkb:Q9GZQ8) physically interacts (MI:0915) with PINK1 (uniprotkb:Q9BXM7) by anti bait coimmunoprecipitation (MI:0006)
- Subjects
MITOCHONDRIA; GENETIC mutation; PARKINSON'S disease; GENE expression; FLUORESCENCE microscopy; FIBROBLASTS; GENE targeting
- Publication
FEBS Letters, 2010, Vol 584, Issue 6, p1073
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2010.02.016