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- Title
Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells
- Authors
Sengupta, Rajib; Billiar, Timothy R.; Atkins, James L.; Kagan, Valerian E.; Stoyanovsky, Detcho A.
- Abstract
Abstract: Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.
- Subjects
NITRIC oxide; LIPOIC acid; THIOREDOXIN; GLUTATHIONE; ALCOHOL dehydrogenase; PROTEIN S; DITHIOLE
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3525
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.10.016