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- Title
Reversible dissociation of flavin mononucleotide from the mammalian membrane-bound NADH:ubiquinone oxidoreductase (complex I)
- Authors
Gostimskaya, Irina S.; Grivennikova, Vera G.; Cecchini, Gary; Vinogradov, Andrei D.
- Abstract
Abstract: Conditions for the reversible dissociation of flavin mononucleotide (FMN) from the membrane-bound mitochondrial NADH:ubiquinone oxidoreductase (complex I) are described. The catalytic activities of the enzyme, i.e. rotenone-insensitive NADH:hexaammineruthenium III reductase and rotenone-sensitive NADH:quinone reductase decline when bovine heart submitochondrial particles are incubated with NADH in the presence of rotenone or cyanide at alkaline pH. FMN protects and fully restores the NADH-induced inactivation whereas riboflavin and flavin adenine dinucleotide do not. The data show that the reduction of complex I significantly weakens the binding of FMN to protein thus resulting in its dissociation when the concentration of holoenzyme is comparable with K d (∼10−8 M at pH 10.0).
- Subjects
FLAVINS; MONONUCLEOSIS; MITOCHONDRIAL membranes; OXIDOREDUCTASES
- Publication
FEBS Letters, 2007, Vol 581, Issue 30, p5803
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.11.048