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- Title
Simultaneous Generation of Cylomegalovirus- Specific CD8<sup>+</sup> and CD4<sup>+</sup> T Lymphocytes by Use of Dendritic Cells Comodified with pp65 mRNA and pp65 Protein.
- Authors
Carlsson, Björn; Mingyan Hou; Giandomenico, Valeria; Nilsson, Berith; Tötterman, Thomas H.; Essand, Magnus
- Abstract
Cytomegalovirus (CMV) disease remains a severe complication in patients who have undergone transplantation. Viremia can be prevented and treated by the adoptive transfer of donor-derived CMV-directed T cells. To ensure long-term protection against CMV disease, it is important to transfer CMV antigen-specific T cells that represent both the CD8+ and the CD4+ subsets. In the present study, we used as stimulators dendritic cells (DCs) that were electroporated with in vitro-transcribed 5'-capped polyadenylated messenger RNA (mRNA) that encoded the CMV pp65 protein (i.e., pp65 mRNA). These DCs could efficiently activate CMV- directed CD8+ T cells, as assayed by tetramer staining, interferon-γ production, and cytolytic activity. We also used DCs that were pulsed with a recombinant pp65 protein to activate CMV-directed CD4+ T cells. When DCs were comodified with pp65 mRNA and pp65 protein, large numbers of CMV-directed CD8+ and CD4+ T cells were generated simultaneously. The approach outlined in the present study can be adapted for a clinical protocol that circumvents potential virus-related biohazards and is available to all patients independently of their human leukocyte antigen haplotype.
- Subjects
CYTOMEGALOVIRUS diseases; DENDRITIC cells; PROTEINS; T cells; LYMPHOCYTES; ANTINEOPLASTIC agents
- Publication
Journal of Infectious Diseases, 2005, Vol 192, Issue 11, p1912
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1086/497700