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- Title
The effect of taurine on hepatic steatosis induced by thioacetamide in zebrafish (Danio rerio).
- Authors
Hammes TO; Pedroso GL; Hartmann CR; Escobar TD; Fracasso LB; da Rosa DP; Marroni NP; Porawski M; da Silveira TR; Hammes, Thais Ortiz; Pedroso, Gabriela Lima; Hartmann, Carolina Rigatti; Escobar, Thayssa Dalla Costa; Fracasso, Laisa Beduschi; da Rosa, Darlan Pase; Marroni, Norma Possa; Porawski, Marilene; da Silveira, Themis Reverbel
- Abstract
<bold>Background: </bold>Nonalcoholic fatty liver disease is one of the most prevalent forms of chronic liver disease in the Western world. Taurine is a conditionally essential amino acid in humans that may be a promising therapy for treating this disease.<bold>Aim: </bold>To evaluate the effect of taurine on hepatic steatosis induced by thioacetamide in Danio rerio.<bold>Methods: </bold>Animals were divided into four groups: control (20 μl of saline solution), taurine (1,000 mg/kg), thioacetamide (300 mg/kg), and the taurine-thioacetamide group (1,000 + 300 mg/kg). Thioacetamide was injected intraperitoneally three times a week for 2 weeks. The mRNA expression, lipoperoxidation, antioxidant enzymatic activity, and histological analyses were evaluated in the liver and the triglyceride content was assessed in the serum.<bold>Results: </bold>Thioacetamide injection induced steatosis, as indicated by histological analyses. The lipoperoxidation showed significant lipid damage in the thioacetamide group compared to the taurine-thioacetamide group (p < 0.001). Superoxide dismutase (SOD) activity in the taurine-thioacetamide group (5.95 ± 0.40) was significantly increased compared to the thioacetamide group (4.14 ± 0.18 U SOD/mg of protein) (p < 0.001). The mRNA expression of SIRT1 (0.5-fold) and Adiponectin receptor 2 (0.39-fold) were lower in the thioacetamide group than the control (p < 0.05). TNF-α mRNA expression was 6.4-fold higher in the thioacetamide group than the control (p < 0.05). SIRT1 mRNA expression was 2.6-fold higher in the taurine-thioacetamide group than in the thioacetamide group.<bold>Conclusions: </bold>Taurine seems to improve hepatic steatosis by reducing oxidative stress and increasing SIRT1 expression.
- Publication
Digestive Diseases & Sciences, 2012, Vol 57, Issue 3, p675
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-011-1931-4