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- Title
Cordycepin Induces Apoptosis through JNK-Mediated Caspase Activation in Human OEC-M1 Oral Cancer Cells.
- Authors
Tung, Kuo-Lung; Wu, Su-Zhen; Yang, Chun-Chuan; Chang, Hong-Yi; Chang, Chun-Sheng; Wang, Yan-Hsiung; Huang, Bu-Miin; Lan, Yu-Yan
- Abstract
Cordycepin, a bioactive compound extracted from Cordyceps sinensis, can induce apoptosis in human OEC-M1 oral cancer cells. However, the exact mechanism is still unclear. The present study aimed to investigate the underlying mechanism of cordycepin-induced apoptosis in OEC-M1 cells. Following treatment with cordycepin, apoptosis was examined and quantified using a DNA laddering assay and a cytokeratin 18 fragment enzyme-linked immunosorbent assay, respectively. Expressions of mitogen-activated protein kinases (MAPKs) and apoptosis-related proteins were detected by the western blot analysis. Our results show that a pan-caspase inhibitor, Z-VAD-FMK, could significantly inhibit cordycepin-induced apoptosis in OEC-M1 cells. In addition, treatment with cordycepin not only activated caspase-8, caspase-9, and caspase-3 but also induced Bid and poly ADP-ribose polymerase cleavages. Furthermore, cordycepin also induced the activation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase, and p38 MAPKs. Among MAPKs, activation of JNK solely contributed to cordycepin-induced apoptosis with the activation of caspase-8, caspase-9, and caspase-3 and cleavage of PARP. Taken together, the present study demonstrated that cordycepin activated JNK and caspase pathways to induce apoptosis in OEC-M1 cells.
- Subjects
DNA analysis; MOUTH tumors; WESTERN immunoblotting; FUNGI; ORGANIC compounds; APOPTOSIS; HEAD &; neck cancer; ENZYME-linked immunosorbent assay; GENE expression profiling; TISSUE extracts; MITOGEN-activated protein kinases; CELL lines; CASPASES; SQUAMOUS cell carcinoma
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2022, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2022/1842363