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- Title
Resistin-binding peptide antagonizes role of resistin on white adipose tissue.
- Authors
Nan Gu; Shu-ping Han; Li Fei; Xiao-qin Pan; Mei Guo; Rong-hua Chen; Xi-rong Guo
- Abstract
Aim: To investigate the direct effects of resistin and resistin-binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue. Methods: Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations(1×10-12mol/L, 1×10-10mol/L, 1×10-8mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-α and adiponectin were detected by ELISA kit and RT-PCR respectively. Results: The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10-8mol/L RBP. The levels of protein secretion and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations. Conclusion: RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.
- Subjects
PEPTIDES; ADIPOSE tissues; LIPID metabolism; ENDOCRINE function tests; DIAGNOSIS of endocrine diseases; FATTY acids; GENE expression; ENZYME-linked immunosorbent assay
- Publication
Acta Pharmacologica Sinica, 2007, Vol 28, Issue 2, p221
- ISSN
1671-4083
- Publication type
Article
- DOI
10.1111/j.1745-7254.2007.00480.x