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- Title
Enhancing Human Glycoprotein Hormones Production in CHO Cells Using Heterologous Beta-Chain Signal Peptides.
- Authors
Sinegubova, M. V.; Kolesov, D. E.; Dayanova, L. K.; Vorobiev, I. I.; Orlova, N. A.
- Abstract
We studied the influence of heterologous signal peptides in the β-chains of glycoprotein hormones on the biosynthesis of these hormones in a transiently transfected culture of Chinese hamster ovary cells CHO S. When the natural signal peptides of the β-chains were replaced with the heterologous signal peptide of human serum albumin, cell productivity was increased 2–2.5 times for human luteinizing hormone, human chorionic gonadotropin, and human thyroid-stimulating hormone, but not for human follicle-stimulating hormone. No significant increase in cell productivity was observed for human azurocidin signal peptide and human glycoprotein hormone α-chain signal peptide. The used approach allows quick assessing the effect of heterologous signal peptides on the biosynthesis of heterodimeric proteins of various classes.
- Subjects
SIGNAL peptides; CHO cell; GLYCOPROTEIN hormones; CHORIONIC gonadotropins; PEPTIDES
- Publication
Doklady Biochemistry & Biophysics, 2024, Vol 514, Issue 1, p1
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672923700576