We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Reconstitution of Biosynthetic Machinery for the Synthesis of the Highly Elaborated Indole Diterpene Penitrem.
- Authors
Chengwei Liu; Koichi Tagami; Atsushi Minami; Tomoyuki Matsumoto; Frisvad, Jens Christian; Hideyuki Suzuki; Jun Ishikawa; Katsuya Gomi; Hideaki Oikawa
- Abstract
Penitrem A is one of the most elaborated members of the fungal indole diterpenes. Two separate penitrem gene clusters were identified using genomic and RNA sequencing data, and 13 out of 17 transformations in the penitrem biosynthesis were elucidated by heterologous reconstitution of the relevant genes. These reactions involve 1) a prenylationinitiated cationic cyclization to install the bicyclo[3.2.0]heptane skeleton (PtmE), 2)a two-step P450-catalyzed oxidative processes forming the unique tricyclic penitrem skeleton (PtmK and PtmU), and 3) five sequential oxidative transformations (PtmKULNJ). Importantly, without conventional gene disruption, reconstitution of the biosynthetic machinery provided sufficient data to determine the pathway. It was thus demonstrated that the Aspergillus oryzae reconstitution system is a powerful method for studying the biosynthesis of complex natural products.
- Subjects
INDOLE; DITERPENES; RNA sequencing; RING formation (Chemistry); BIOSYNTHESIS; BIOCHEMICAL research
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 19, p5840
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/anie.201501072