We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
High cell density cultivation of Brevibacterium linens and formation of proteinases and lipase.
- Authors
Adamitsch, Bernhard F.; Karner, Ferdinand; Hampel, Werner A.
- Abstract
Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed of nutrients. The maximal activities of l-leucine aminopeptidase and cell-associated proteinase were 286 U l-1 and 202 U l-1, respectively. The cell-associated lipolytic activity exhibited a strong and sudden increase at 46 h, resulting in a maximum of 9.5 U g-1 dry wt; thus the volumetric productivity of proteolytic and lipolytic activity was 4220 U l-1 h-1 and 7.3 U l-1 h-1, respectively.
- Subjects
BREVIBACTERIUM; CORYNEBACTERIUM; PROTEOLYTIC enzymes; PLANT fibers; AMMONIUM sulfate; AMINO acids; AMINOPEPTIDASES
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 9, p705
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1023402529882