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- Title
A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors.
- Authors
Ho Je, Yeon; Hee Chang, Jin; Young Choi, Jae; Yul Roh, Jong; Rae Jin, Byung; O'Reilly, David R.; Kwon Kang, Seok
- Abstract
A novel baculovirus genome has been generated that can be maintained in Escherichia coli and which facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. This genome, designated bAcGOZA, lacks a portion of the essential ORF1629 gene but includes a miniF replicon and selectable kanamycin-resistance marker. The bAcGOZA can replicate only in E. coli and can be rescued by recombination with a transfer vector containing an intact ORF1629. There is no background of non-recombinant virus.
- Subjects
ESCHERICHIA coli; GENETIC vectors; ENTEROBACTERIACEAE; RECOMBINANT microorganisms; GENETICS; VIRUSES
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 8, p575
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1010301404445