We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhancement of penicillin G hydrolysis using penicillin acylase to 6-aminopenicillanic acid by simultaneous chromatographic separation.
- Authors
Wu, J. C.; He, Z. M.; Han, Z. W.; Yu, K. T.
- Abstract
Penicillin G (Pen-G) was hydrolyzed to 6-aminopenicillanic acid (6-APA) and phenylacetic acid (PAA) in a chromatographic reactor-separator using the mixture of immobilized Escherichia coli cells and a macroporous adsorbent as stationary phase and a phosphate buffer of pH 7.8 as eluant. Pen-G conversion of 98% was observed without adjustment of the eluant pH due to the effective separation of 6-APA from Pen-G and PAA. At a sample load of 600 mg Pen-G, the volume overload gave higher Pen-G conversion (86%) than the mass overload (68%), while their difference in product resolution (0.9 and 1.0, respectively) was insignificant.
- Subjects
PENICILLIN; PHENYLACETIC acid; CHROMATOGRAPHIC analysis; ESCHERICHIA coli; HYDROLYSIS; ANTIBACTERIAL agents
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 24, p1959
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1026706111815