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- Title
Determination of the Toxicity Preferences of Ocular Drug Delivery System by Comparing Two Different Toxicity Bioassays.
- Authors
Uner, Burcu; Durgun, Meltem Ezgi; Ozdemir, Samet; Tas, Cetin; Uner, Melike; Ozsoy, Yildiz
- Abstract
Ocular drug delivery methods are highly favored for boosting bioavailability, patient compliance, and lower adverse effects and dose frequency. In addition to preventing adverse effects from the active ingredient, the parts of drug delivery systems must be nontoxic and nonallergic as well. Mitochondrial toxicity test (MTT) and Hen's egg chorioallantois membrane (HET-CAM) assay are the most often utilized tests based on this dilemma. The toxicity of loteprednol etabonate loaded solid lipid nanoparticles, lipid nanostructured carriers, and nanoemulsion were compared. Oleic acid, Precirol®ATO5, and Pluronic® F68 were used in the preparation. Their toxicities were evaluated by using two different toxicity tests (MTT and HET-CAM). The results suggest that there are no significant differences between the HET-CAM and MTT assays. It is noteworthy that the HET-CAM assay offers a more cost-effective and environmentally friendly alternative to the MTT assay, as it does not require cell culture and generates less toxic waste. This information may be useful to consider when selecting between the two assays.
- Subjects
DRUG delivery systems; OCULAR toxicology; TOXICITY testing; EGGS; HAZARDOUS wastes; ITRACONAZOLE
- Publication
Assay & Drug Development Technologies, 2023, Vol 21, Issue 7, p337
- ISSN
1540-658X
- Publication type
Article
- DOI
10.1089/adt.2023.058